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Background: Human identification is necessary for personal, social, and legal reasons. Some methods of personal identification include anthropometry, dactyloscopy, DNA test, fingerprinting, sex determination, estimation of age, measurement of height, post-mortem reports, blood groups, and lip print. The aim of the present study was to assess the correlation of lip print pattern with gender and ABO blood group. Materials and Methods: A study was conducted among 233 undergraduate dental students of Visnagar of Gujarat state, India during the month of May, 2014. Lipstick was used to record lip print of each subject. To identify the blood group of study subjects, a drop blood of each subject was placed on a slide and mixed with anti-A, anti-B, and anti-Rh sera. Results: Type II (A branched groove) pattern was to be found more in both male (44.2%) and female (31.5%). Type II (A branched groove) pattern was to be found more among study subjects with blood Group A (29.4%), B (37.3%), and O (37.1%) blood group. No correlation was found between lip print and blood group. Conclusion: The present study did not show any statistical correlation of lip print pattern with gender and ABO blood groups.